RANTES mRNA analysis in dendritic cell-platelet co-culture. Dendritic cells (DCs) were cultured for 48 h with sCD154 or with platelets (PLTs) either in two 0.4-μm pore-sized filter-separated compartments or in the same compartment. As culture negative controls, DCs or PLTs were cultured alone for 48 h (untreated DCs, untreated PLTs). The positive PCR control consisted of a synthetic double-stranded DNA provided by the manufacturer, the negative PCR control consisted of water instead of cDNA template for PCR. Data are representative of three independent experiments.