Figure 4

Granulocytic functionality. Phagoburst results are shown in response to PMA, fMLP and E. coli of HPC expanded in IL1-stimulated EC and following further differentiation by G-CSF in comparison to granulocytes differentiated by cytokines alone. A) HPC differentiated following expansion in IL1-stimulated EC supernatant; B) HPC differentiated following expansion in IL1-stimulated EC supernatant and overnight storage in human serum prior to analysis; C) HPC differentiated in a cytokine combination of erythropoietin, SCF and G-CSF without endothelial supernatant. Shown is one representative result of eight independent experiments. Shaded histograms: sample fluorescence; white line: negative control. PMA: phorbol 12-myristate 13-acetate; E. coli: Escherichia coli; fMLP: N-formyl-methionyl-leucyl-phenylalanin.