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Table 1 Cell expansion in IL-stimulated endothelial supernatant following a period of 7 and 14 days and flow cytometric profile on day 7.

From: Hematopoietic progenitor cells and interleukin-stimulated endothelium: expansion and differentiation of myeloid precursors

 

Concentration (U/ml)

Fold increases

Flow cytometry

  

7 days

14 days

CD33, 34, 45, 14, 16, 133

After isolation

N/A

N/A

Control

Non-stimulated

1.2 ± 0.13

6.4 ± 1.2

 

0.1% BSA

1.3 ± 0.17

6.8 ± 1.6

IL-1β

1

5 ± 1a

6.3 ± 0.12

N/A

 

10

13 ± 2.2a,b

59.1 ± 13.3a,b

 

100

15.8 ± 2.5a,b

136.8 ± 23.3a,b

 

1,000

19.4 ± 8.6a,b

142.7 ± 23.5a,b

 

10,000

10.5 ± 2.8a,b

97 ± 22.4a,b

IL-3

10

11.8 ± 1.6a,b

71.2 ± 10.5a,b

 

100

15.5 ± 1.6a,b

82.9 ± 14.5a,b

 

1,000

15.2 ± 1.4a,b

79.6 ± 9.9a,b

IL-6

10

1.5 ± 0.51

2.8 ± 0.58

 

100

1.2 ± 0.22

5.2 ± 1.2

 

1,000

1.3 ± 0.21

4.6 ± 0.77

  1. Fold expansions were determined following a period of seven and fourteen days. Percentage of CD33, 34, 45, 14, 16 and CD133 positivity are depicted as circles (: negative, less than 10%; quarter circle: 10 – 25% positivity; half circle: 25 – 50% positivity; more than 75% positive cells).
  2. a: highly significant different compared to non-stimulated supernatant (p < 0.001); b: highly significant different compared to BSA supernatant (p < 0.001). Shown are mean results ± SE of twelve independent experiments. N/A: not applicable.