Hematopoietic progenitor cells and interleukin-stimulated endothelium: expansion and differentiation of myeloid precursors

Table 2 Colony forming activity of HPC expanded in IL-stimulated EC supernatant for one week.

	Concentration (U/ml)	BFU-E (×10³)	CFU-GM (×10³)	CFU-Mix (×10³)	PE (%)
Post isolation (5 × 10⁴ cells)	N/A	2.5 ± 0.18	3.3 ± 0.48	0.16 ± 0.02	7.9 ± 0.57
Controls
- No stimulus	N/A	2 ± 0.28	2.7 ± 0.46	0.36 ± 0.14	6.4 ± 0.67
- BSA	0.1%	3 ± 0.46	4.3 ± 0.74	0.14 ± 0.05	4.5 ± 1.1^c
IL-1β	10	3.6 ± 1.1^a	1.8 ± 0.56	0.16 ± 0.07	0.97 ± 0.06^a,b,c
	100	5.2 ± 1.6^a,c	2.4 ± 0.82	0.22 ± 0.08	1 ± 0.06^a.b.c
	1,000	6.4 ± 1.2^a,b,c	2.4 ± 0.43	0.22 ± 0.08	1.1 ± 0.08^a,b,c
IL-3	10	10 ± 3.3^a,b,c	3.1 ± 1	2.2 ± 0.74^a,b,c	3.9 ± 1.3^c
	100	9.5 ± 3.2^a,b,c	3.1 ± 1	2.1 ± 0.71^a,b,c	1.6 ± 0.54^a,c
	1,000	12.8 ± 4.3^a,b,c	6.3 ± 2.1^a,c	5 ± 1.7^a,b,c	1.7 ± 0.58^a,c
IL-6	10	1.5 ± 0.34^c	1.5 ± 0.37^b>,c	0.28 ± 0.11	8.2 ± 1.7
	100	2.1 ± 0.4	3.1 ± 0.67	0.18 ± 0.07	8.9 ± 1.4
	1,000	3.1 ± 0.58	4 ± 0.69	0.24 ± 0.08	11.8 ± 1.2^a,b,c

Hematopoietic colony formation was determined after fourteen days in semisolid methylcellulose cultures supplemented with erythropoietin, GM-CSF, IL-3 and stem cell factor. Total colonies were defined by multiplying counted colonies with the number of expanded cells divided by the number of input cells. Mean values ± SE from four to nine independent experiments conducted in triplicate. BFU-E: burst-forming unit erythrocyte; CFU-GM: colony-forming unit granulocyte macrophage; CFU-Mix; mixed colony-forming unit (granulocyte, erythrocyte, megakaryocyte, macrophage); PE: plating efficiency; N/A: not applicable; BSA: bovine serum albumin
a: significant different compared to non-stimulated supernatant; b: significant different compared to BSA supernatant; c: significant different compared to freshly isolated CD34(+) cells.

ISSN: 1471-2172