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Table 3 Cobblestone area and long-term culture initiating cells (LTC-IC) of HPC post-isolation and of cells cultured in IL-stimulated EC supernatant for one week.

From: Hematopoietic progenitor cells and interleukin-stimulated endothelium: expansion and differentiation of myeloid precursors

 

Concentration (U/ml)

CAFC

LTC-IC

  

2-week

5-week

 

Post isolation

N/A

4.9 ± 0.73

23.2 ± 4.2

16.3 ± 3

Control

No addition

11.6 ± 2.5c

16.8 ± 4.5

17 ± 4.3

 

0.1% BSA

9.6 ± 2.8

17.8 ± 4.5

16.2 ± 4.2

IL-1β

10

3.7 ± 1.1

5.4 ± 2.4a,c

7 ± 2.2

 

100

4.9 ± 0.95

3.4 ± 1.2a,b,c

6.6 ± 2.3

 

1,000

5.6 ± 0.92

0.45 ± 0.2a,c

3.7 ± 2.2c

 

10,000

19.2 ± 7.1b,c

0.38 ± 0.17a,b,c

n.d.

IL-3

10

3.6 ± 1.1a,c

8.1 ± 2a,c

3.2 ± 2c

 

100

4.3 ± 1.5a

2.8 ± 0.58a,b,c

2.3 ± 0.59c

 

1,000

2.2 ± 0.5a,c

4 ± 0.88a,b,c

1.8 ± 0.61c

IL-6

10

3.6 ± 1.1

9 ± 2

11 ± 3.2

 

100

4.3 ± 1.5

20.9 ± 4.1

13.2 ± 6.3

 

1,000

2.2 ± 0.5a,b

15.7 ± 3.3

8.5 ± 3.4

  1. Freshly isolated and expanded HPC were cultured on the murine bone marrow stroma cell line MS-5 and scored for cobblestone-area formation after two and five weeks. LTC-IC were scored by replating 5-week CAFC in methylcellulose for secondary colony formation. Shown are mean results ± SD of three independent experiments in triplicate; a: significant different compared to non-stimulated supernatant (p < 0.05); b: significant different compared to BSA supernatant (p < 0.05); c: significant different compared to freshly isolated CD34(+) cells.