Figure 3
TGase activity is low in cells migrating out of the Hpt. Hematopoietic tissues were cultured on coverslips for one week, and labelled with 1 mM BPNH2 overnight. A-C. Migrating (spread) cells (arrows) can be seen at distance from the centre of the tissue; D-F. TGase activity in migrating cells; G-I. TGase activity in cells close to the centre of the tissue. Interference contrast (D, G); TGase activity (E, H); Nuclear staining was performed with DAPI (F, I). Bar is 40 μm.