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Figure 5 | BMC Immunology

Figure 5

From: Transglutaminase activity in the hematopoietic tissue of a crustacean, Pacifastacus leniusculus, importance in hemocyte homeostasis

Figure 5

TGase activity of HSCs does not prevent its proliferation. Hpt cells were cultured on coverslips, and maintained in six well plates. The cells were labeled with 1 mM 5-(biotinamindo)-pentylamine (BPNH2; Molecular Probes, Pierce) as a substrate for TGase and 10 μM BrdU (Sigma) for 2 or 16 hours. The in situ TGase activity was stained by streptavidin-Cy5, BrdU incorporation assay was measured by staining with anti-BrdU-FITC. A-D. Control cells incubated without BrdU and BPNH2.E-H. Cells incubated with BrdU and BPNH2 for 2 hours. I-L. Cells incubated with BrdU and BPNH2 for 16 hours. M-P. Cell surface TGase activity detected in non-permeabilized cells after incubation with BPNH2 for 16 hours.

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