Figure 5From: Transglutaminase activity in the hematopoietic tissue of a crustacean, Pacifastacus leniusculus, importance in hemocyte homeostasisTGase activity of HSCs does not prevent its proliferation. Hpt cells were cultured on coverslips, and maintained in six well plates. The cells were labeled with 1 mM 5-(biotinamindo)-pentylamine (BPNH2; Molecular Probes, Pierce) as a substrate for TGase and 10 μM BrdU (Sigma) for 2 or 16 hours. The in situ TGase activity was stained by streptavidin-Cy5, BrdU incorporation assay was measured by staining with anti-BrdU-FITC. A-D. Control cells incubated without BrdU and BPNH2.E-H. Cells incubated with BrdU and BPNH2 for 2 hours. I-L. Cells incubated with BrdU and BPNH2 for 16 hours. M-P. Cell surface TGase activity detected in non-permeabilized cells after incubation with BPNH2 for 16 hours.Back to article page