Figure 7
Astakine treatment results in decreased TGase activity in the Hpt cells. Hpt cells were seeded on coverslips, and maintained in culture dishes, and the medium supplied was added with or without astakine (a crayfish prokineticin domain-containing protein) [21]. At day 4 (E-T) and day 7 (U-X), the cells were incubated with 1 mM BPNH2 in medium two hours at room temperature, and visualized using the streptavidin-FITC conjugate. Nuclear staining was performed with propidium iodide. A-D. Non-permeabilized Hpt cells cultured for 4 days without BPNH2 were used as a staining control. E-H. Non-permeabilized Hpt cells cultured for 4 days without astakine showing surface TGase activity. I-L. Non-permeabilized Hpt cells cultured for 4 days with astakine showing no surface TGase activity. M-P. Permeabilized Hpt cells cultured for 4 days without astakine. Q-T. Permeabilized Hpt cells cultured for 4 days with astakine. U-X. Permeabilized Hpt cells cultured for 7 days with astakine showing no TGase activity. Bar is 40 μm.