Figure 3

Distinct ψ in different subsets of PBL. (A) Dot-plot of control PBL stained with anti-CD3 (y-axis) and anti-CD45RO (x-axis). The CD3⁺/CD45RO^- and CD3⁺/CD45RO⁺ sets are depicted in the upper left (R2) and upper right gate (R3), respectively. (B) Dot-plot of the CD45RO sub-population according to the oxonol fluorescence distribution. Numbers displayed inside the dot-plot represent the percentage of cells contained in each quadrant. (C) Histograms distributions of oxonol fluorescence in control PBL cells gated on R2 (red) or R3 (black). Numbers depicted represent the ψ values. Data are representative of six experiments.