Effects of MgTX or high [K+]
on subsets of CD3+ cells. Histograms distribution of oxonol fluorescence in CD3+/CD45RO- and CD3+/CD45RO+ untreated cells (gray lines) and treated with MgTX (full lines) or high [K+]e (dashed lines). The proportions of CD3+/CD45RO- and CD3+/CD45RO+ were 30% and 60% (A), 60% and 30% (B) and 45% and 35% (C) for the CD45RO- and CD45RO+ populations of the total CD3+, respectively. The black marker indicates depolarized cells as determined by [K+]e treatment. Data are representative of six experiments.