Figure 6

The effects of oxidants and thioredoxin on cytokine production in Jurkat T cells: Thioredoxin up-regulates IFN-γ production and counteracts Th2 cytokine bias caused by oxidants. Jurkat T cells were either mock-transfected with empty vector (Mock) or transfected with thioredoxin expression construct (Trx) and then incubated for 24 h. The cells were then pretreated with hydrogen peroxide (400 μM) for 1 h, after which they were incubated with media alone or with human anti-CD3 (1 μg/ml) plus anti-CD28 (1 μg/ml) to stimulate cytokine production for 4 h. RT-PCR was then performed to determine the mRNA levels of IL-4, IFN-γ, and thioredoxin.