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Table 1 FACS analysis of lymphocyte subpopulations in conventional and germ-free Balb/c mice fed either a low LPS diet (AIN-93G) or a LPS-rich diet (ST1)

From: Gut microbiota and lipopolysaccharide content of the diet influence development of regulatory T cells: studies in germ-free mice

  

Number of positive lymphocytes (× 106)

Lymphoid organ/tissue

Subpopulation

CV ST1

GF ST1

GF AIN

Peyer's patches

Total

4.69 ± 0.16

1.57 ± 0.19

0.67 ± 0.09

 

CD19+

2.93 ± 0.10

1.00 ± 0.12

0.40 ± 0.05

 

CD3+

1.38 ± 0.05

0.44 ± 0.05

0.21 ± 0.03

 

CD4+

0.95 ± 0.03

0.33 ± 0.04

0.16 ± 0.02

 

CD8+

0.27 ± 0.01

0.08 ± 0.01

0.04 ± 0.01

 

CD4+Foxp3+

0.15 ± 0.01

0.04 ± 0.01

0.02 ± 0.00

 

CD8+Foxp3+

0.02 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

Mesenteric lymph nodes

Total

17.81 ± 0.69

7.82 ± 0.57

4.96 ± 0.96

 

CD19+

4.39 ± 0.17

2.96 ± 0.21

2.48 ± 0.48

 

CD3+

12.10 ± 0.47

4.70 ± 0.34

2.36 ± 0.46

 

CD4+

8.70 ± 0.34

3.40 ± 0.25

1.53 ± 0.30

 

CD8+

2.41 ± 0.09

1.03 ± 0.07

0.64 ± 0.12

 

CD4+Foxp3+

1.42 ± 0.05

0.48 ± 0.04

0.22 ± 0.04

 

CD8+Foxp3+

0.06 ± 0.00

0.02 ± 0.00

0.01 ± 0.00

Spleen

Total

83.14 ± 5.28

62.48 ± 3.46

66.21 ± 4.12

 

CD19+

46.14 ± 2.93

35.59 ± 1.97

36.44 ± 2.27

 

CD3+

24.74 ± 1.57

20.54 ± 1.14

16.93 ± 1.05

 

CD4+

16.32 ± 1.04

12.35 ± 0.68

11.18 ± 0.70

 

CD8+

7.00 ± 0.44

5.36 ± 0.30

5.31 ± 0.33

 

CD4+Foxp3+

3.03 ± 0.19

2.16 ± 0.12

2.27 ± 0.14

 

CD8+Foxp3+

0.12 ± 0.01

0.12 ± 0.01

0.11 ± 0.01

Peritoneal cells

Total

2.55 ± 0.41

2.65 ± 0.06

2.50 ± 0.35

 

CD19+

1.80 ± 0.29

2.22 ± 0.05

2.19 ± 0.30

 

CD3+

0.11 ± 0.02

0.08 ± 0.00

0.07 ± 0.01

 

CD4+

0.22 ± 0.04

0.18 ± 0.00

0.16 ± 0.02

 

CD8+

0.06 ± 0.01

0.06 ± 0.00

0.05 ± 0.01

 

CD4+Foxp3+

0.03 ± 0.01

0.03 ± 0.00

0.03 ± 0.00

 

CD8+Foxp3+

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

Thymus

Total

207.03 ± 9.23

205.76 ± 8.46

205.76 ± 7.98

 

CD4-CD8-

8.70 ± 0.30

10.72 ± 0.50

11.87 ± 0.20

 

CD4+CD8+

155.27 ± 8.13

151.59 ± 7.26

146.52 ± 7.18

 

CD4+CD8-

30.23 ± 1.82

28.81 ± 1.72

31.39 ± 2.39

 

CD8+CD4-

13.04 ± 0.67

11.93 ± 0.57

9.88 ± 0.57

 

CD4+Foxp3+

1.35 ± 0.05

1.25 ± 0.03

1.15 ± 0.02

 

CD8+Foxp3+

0.66 ± 0.02

0.66 ± 0.02

0.65 ± 0.01

  1. The single cell suspensions were stained with the monoclonal antibodies as described under "Methods" and analyzed by FACS. PP and MLN cell numbers represent the overall cell pool isolated from one mouse. Data is a summary of at least four independent experiments (at least 10 mice/group) and values represent the mean ± SE.