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Table 1 FACS analysis of lymphocyte subpopulations in conventional and germ-free Balb/c mice fed either a low LPS diet (AIN-93G) or a LPS-rich diet (ST1)

From: Gut microbiota and lipopolysaccharide content of the diet influence development of regulatory T cells: studies in germ-free mice

   Number of positive lymphocytes (× 106)
Lymphoid organ/tissue Subpopulation CV ST1 GF ST1 GF AIN
Peyer's patches Total 4.69 ± 0.16 1.57 ± 0.19 0.67 ± 0.09
  CD19+ 2.93 ± 0.10 1.00 ± 0.12 0.40 ± 0.05
  CD3+ 1.38 ± 0.05 0.44 ± 0.05 0.21 ± 0.03
  CD4+ 0.95 ± 0.03 0.33 ± 0.04 0.16 ± 0.02
  CD8+ 0.27 ± 0.01 0.08 ± 0.01 0.04 ± 0.01
  CD4+Foxp3+ 0.15 ± 0.01 0.04 ± 0.01 0.02 ± 0.00
  CD8+Foxp3+ 0.02 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
Mesenteric lymph nodes Total 17.81 ± 0.69 7.82 ± 0.57 4.96 ± 0.96
  CD19+ 4.39 ± 0.17 2.96 ± 0.21 2.48 ± 0.48
  CD3+ 12.10 ± 0.47 4.70 ± 0.34 2.36 ± 0.46
  CD4+ 8.70 ± 0.34 3.40 ± 0.25 1.53 ± 0.30
  CD8+ 2.41 ± 0.09 1.03 ± 0.07 0.64 ± 0.12
  CD4+Foxp3+ 1.42 ± 0.05 0.48 ± 0.04 0.22 ± 0.04
  CD8+Foxp3+ 0.06 ± 0.00 0.02 ± 0.00 0.01 ± 0.00
Spleen Total 83.14 ± 5.28 62.48 ± 3.46 66.21 ± 4.12
  CD19+ 46.14 ± 2.93 35.59 ± 1.97 36.44 ± 2.27
  CD3+ 24.74 ± 1.57 20.54 ± 1.14 16.93 ± 1.05
  CD4+ 16.32 ± 1.04 12.35 ± 0.68 11.18 ± 0.70
  CD8+ 7.00 ± 0.44 5.36 ± 0.30 5.31 ± 0.33
  CD4+Foxp3+ 3.03 ± 0.19 2.16 ± 0.12 2.27 ± 0.14
  CD8+Foxp3+ 0.12 ± 0.01 0.12 ± 0.01 0.11 ± 0.01
Peritoneal cells Total 2.55 ± 0.41 2.65 ± 0.06 2.50 ± 0.35
  CD19+ 1.80 ± 0.29 2.22 ± 0.05 2.19 ± 0.30
  CD3+ 0.11 ± 0.02 0.08 ± 0.00 0.07 ± 0.01
  CD4+ 0.22 ± 0.04 0.18 ± 0.00 0.16 ± 0.02
  CD8+ 0.06 ± 0.01 0.06 ± 0.00 0.05 ± 0.01
  CD4+Foxp3+ 0.03 ± 0.01 0.03 ± 0.00 0.03 ± 0.00
  CD8+Foxp3+ 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
Thymus Total 207.03 ± 9.23 205.76 ± 8.46 205.76 ± 7.98
  CD4-CD8- 8.70 ± 0.30 10.72 ± 0.50 11.87 ± 0.20
  CD4+CD8+ 155.27 ± 8.13 151.59 ± 7.26 146.52 ± 7.18
  CD4+CD8- 30.23 ± 1.82 28.81 ± 1.72 31.39 ± 2.39
  CD8+CD4- 13.04 ± 0.67 11.93 ± 0.57 9.88 ± 0.57
  CD4+Foxp3+ 1.35 ± 0.05 1.25 ± 0.03 1.15 ± 0.02
  CD8+Foxp3+ 0.66 ± 0.02 0.66 ± 0.02 0.65 ± 0.01
  1. The single cell suspensions were stained with the monoclonal antibodies as described under "Methods" and analyzed by FACS. PP and MLN cell numbers represent the overall cell pool isolated from one mouse. Data is a summary of at least four independent experiments (at least 10 mice/group) and values represent the mean ± SE.