Expression analysis of the BS CXC. (A) The expression of BSCXC mRNA relative to b-actin analyzed by real-time PCR in Black Seabream after PolyI:C and LPS challenge. The values are shown as means ± S.E., n = 3. Significant differences between challenged group and control group are indicated by an asterisk (P < 0.01). (B) The plasmid BS CXC-pQE30 was transformed into E. coli M15 cells and induced by ITPG. Bacterial lysates (lane 1) or purified BS rCXC (lanes 3 and 4) were separated on SDS-PAGE and stained by Coomassie brilliant blue R250. Lysate from uninduced cells was used as a control (lane 2). (C) The identity of the BS rCXC was confirmed by Western blotting using the antibody of 6His. Lanes 1 and 2 showed BS rCXC.