Figure 3From: Transcriptional and apoptotic responses of THP-1 cells to challenge with toxigenic, and non-toxigenic Bacillus anthracisReal-Time PCR of single gene treatments confirms trends in microarray expression levels. Y-axis in microarray data (left) represents the fold change in gene expression relative to untreated control samples (labeled control) of THP-1 cells. The Y-axis in Real-Time PCR data (right) represents the fold gene expression in the THP-1 cells within the sample relative to the level of endogenous 18-S gene expression, and was calculated using the 2-ΔΔCT method described by Livak and Schmittgen[69]. Labels within each panel indicate the time post- exposure, the challenge strain and the MOI.Back to article page