CFLAR protein induction in THP-1 cells. A. Western blots of CFLAR induction at MOIs 1:1 and 1:10 at 4 h post infection. B. Inhibition of NF-κB with the inhibitor QNZ during challenge suppresses the production of cFLAR. Samples were taken at 8 h post infection at MOI 1:1. C denotes unchallenged control; S, challenge with Sterne strain; and ΔS, challenge with Δ Sterne strain. Actin is shown as a control.