Analysis of the IL-2 and IL-2R mRNA level in spleen by reverse transcription-PCR. Semi-quantitative detection of IL-2 and IL-2 receptor gene transcription before and after oral administration of live LcZhang with three dosages compared to the gene transcription of β-actin. A: IL-2 gene transcription; B: IL-2 receptor gene transcription. The relative levels of IL-2 and IL-2R mRNA in mouse spleen was calculated and expressed at the percentage of mRNA to β-actin. The control (open column) used normal PEF food with 1.0 ml PBS. Three dosages of bacteria were shown: a low dose means 1 ml × 0.25 × 109 cells/ml (grey column); a medium dose was 1 ml × 0.5 × 109 cells/ml (dark grey column); a high dose was 1 ml × 1.0 × 109 cells/ml (solid column). Data are means ± S.D. (n = 5). Error bars represent S.D. *P < 0.05; **P < 0.01.