Cell surface phenotype of monocyte-derived dendritic cells. Adherent mononuclear cells were cultured for 7 days with GM-CSF and IL-13 with addition of PGE2 for the final 2 days. Cells were then stained with specific monoclonal antibodies or the respective isotype controls and analysed in flow cytometry. Percentage of positive cells for CD1a were 48.4 ± 20.7%, for CD83 39.9 ± 14.5% and for CD209 89.5 ± 8.1%. Shown is one representative of 9 experiments.