Induction of Pam
Cys tolerance in dendritic cells and effect on levels of IRAK1 protein. Day 7 dendritic cells were cultured for additional 2 days with Pam3Cys at 1μg/ml and then washed and stimulated at 10 μg Pam3Cys/ml. A. Cells were lysed at 2 hours and RNA was extracted, transcribed into cDNA and amplified by RT-PCR and data were normalized relative to levels of the house keeping gene alpha-Enolase. Given is the average of 5 experiments. * = p < 0.05 compared to 0/LPS. B. Lysates were prepared at 1 h and were subjected to Western blotting for IRAK-1. One representative of 3 experiments. C. Lysates were prepared at 1 h and were subjected to Western blotting for IRAK-4. One representative of 3 experiments.