Acyl-CoA activity in triglyceride-loaded THP-1 cells treated with IL-1β. Cells were differentiated, incubated for 24 h with VLDL (50 μg/ml), and then incubated in lipoprotein-free media for an additional 24 h with IL-1β. Cells were homogenized and intracellular fractions isolated by differential centrifugation. Enzymatic activity during a five minutes incubation was assayed in fractions containing mitochondria (open circles) or endoplasmic reticulum (closed circles). Data represents mean ± SEM, n = 6, for a representative experiment repeated three times. * = P < 0.05, ** = P < 0.01.