Figure 7

Apolipoprotein E secretion from lipid loaded and cytokine treated cells. Cells were differentiated for four days, lipid loaded for 24 h, then treated with respective cytokine in lipoprotein-free media for an additional 24 h. ApoE content was measured in conditioned media after cytokine treatment. A. ApoE secreted from primary human macrophages after lipid loading and treatment with IL-1β. B. ApoE secreted from primary human macrophages after lipid loading and treatment with TNF-α. C. ApoE secreted from THP-1 cells after lipid loading and treatment with IL-1β. D. ApoE secreted from THP-1 cells after lipid loading and treatment with TNF-α. Data represents mean ± SEM (n = 6) for a representative experiment repeated three times. * = P < 0.05, ** = P < 0.01 compared to cells incubated in absence of TNF-α. Diamonds = control cells (no lipoprotein lipid loading), squares = AgLDL, triangles = VLDL.