Figure 1

Influence of human serum and plasma on the binding of CCL5 to endothelial cells. CCL5 (1 μg/ml) was preincubated with different concentrations of human serum (black bars) or human plasma (grey bars) and subsequently incubated with human microvascular endothelial cells. Dilutions were performed with PBS. Binding of CCL5 to the cell surface was quantified by FACS-analysis and is given as mean fluorescence intensity (MFI). Preincubation of CCL5 with low concentrations of serum significantly enhanced CCL5 binding, while preincubation with high concentrations of serum reduced CCL5 binding. Preincubation of CCL5 with plasma did not result in an increased immobilization of CCL5.