Figure 2

Chondroitinsulfate A enhances the binding of CCL5 to endothelial cells. A) Digestion of serum with chondroinitase ABC abrogates the effects of serum on CCL5 binding. Serum was digested with chondroinitase ABC (grey bars) or left undigested (black bars) and then incubated at different concentrations with human CCL5 (1 μg/ml). Subsequently, binding of CCL5 to human microvascular endothelial cells was quantified by FACS-analysis and is given as mean fluorescence intensity (MFI). Undigested serum reduced or increased the binding of CCL5 in a concentration dependent manner, while digested serum did not alter CCL5 binding. Maximal reduction of CCL5 binding reached statistical significance (p = 0.03) compared to undigested serum. B) Influence of various types of chondroitinsulfate on the binding of CCL5 to human microvascular endothelial cells. CCL5 (1 μg/ml) was preincubated with different concentrations of chondroitinsulfate A (CSA), chondroitinsulfate B (CSB) or chondroitinsulfate C (CSC). Preincubation of CCL5 with low concentrations of CSA results in an significantly (p = 0.01) enhanced binding of CCL5 to endothelial cells, while preincubation of CCL5 with high concentrations of CSA or with CSB and CSC results in a reduced binding of CCL5.