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Figure 1 | BMC Immunology

Figure 1

From: Insulin-like growth factor I promotes cord blood T cell maturation through monocytes and inhibits their apoptosis in part through interleukin-6

Figure 1

The kinetics of CD45 isoform changes for CD4+ T helper cells and CD8+ cytotoxic T cells in (a) CBMC culture; and, (b) purified CD3+ T cell culture. CBMC (a) or CD3+ T cells (b) were incubated for different period with PHA (1 μg/ml --------) alone or PHA + IGF-I (100 ng/ml ——). CD45 isoform expression was determined by three-colour flow cytometry. The kinetics of transition from naïve to mature/memory phenotype in CD4+ and CD8+ cells were similar. In CBMC, IGF-I promoted the PHA-induced maturation of CD45 isoforms. In CD3+ T cells, the maturation process was delayed and IGF-I only slightly promoted the PHA-induced maturation of CD45 isoforms. Results shown are representatives of 5 similar independent cases in each group.

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