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Figure 1 | BMC Immunology

Figure 1

From: Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides

Figure 1

Intra-assay CVs. The mean CV for six replicates was plotted for samples from three donors and two different antigen stimulations in tetramer, CFC, and ELISPOT assays. Circles represent donor 41; triangles, donor 68; and squares, donor 43. Open symbols represent CMV pp65495–503 responses; closed symbols represent CMV pp65 peptide mix responses (CFC and ELISPOT assays only). Note that certain responses were very similar, so some symbols overlap. Error bars represent the SD of 10 times that the six replicates were repeated. The gray zones indicate the area within which a laboratory doing validation could expect their data to lie.

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