Figure 2From: Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptidesSD of each assay. The mean response for each sample (as per Figure 1) was plotted versus the SD of six replicates (intra-assay precision), eight assays on different days (inter-assay precision), or three operators on the same day (inter-operator precision). Circles represent donor 41; triangles, donor 68; and squares, donor 43. Open symbols represent CMV pp65495–503 responses; closed symbols represent CMV pp65 peptide mix responses (CFC and ELISPOT assays only). Error bars in the intra-assay graphs represent the SD of 10 times that the six replicates were repeated. Lines represent linear regression of the combined data (both antigens, where used), with 95% confidence intervals of the regression shown with dotted lines.Back to article page