Figure 3

Comparison of RNA and DNA repertoires. RNA and DNA were extracted from each peripheral blood sample from 8 CLL patients, on which multiplex RT-PCR or PCR was performed respectively and sequenced by MiSeq (250 bp paired-end). A) The percentage of DNA sequences found in each RNA sample. The correlation between the BCR frequency in RNA and functional DNA repertoires (DNA sequences that were found also in the RNA repertoire) for the 8 CLL patients in B) all IgHV gene usage frequencies and C) the low frequency IgHV gene usage frequencies only (<2%). If unequal numbers of RNA molecules per cell significantly skewed the RNA BCR repertoires, then deviation from y = x correlation would be expected.