Figure 4

Transfusion of T cells restored the structure of splenic FRCs and could be blocked by the antibody against the LT-B receptors. Naive T cells were purified and transferred into nude mice every week, after four week Spleen cryostat sections from spleens of BALB/c, nude mice, nude mice + Tcells and nude mice + Tcells + anti-LtBR, mice were stained for CCL21 (red) and ER-TR7(green) (A, Scale bars: 200um). We further analyzed the average fluorescence values of both ER-TR7 and CCL21 (B), RT-qPCR of chemokine expression in whole spleen of BALB/c, nude mice,nude mice + Tcells and nude mice + Tcells + anti-LtBR, (C), chemokine expression in the spleen of BALB/c, nude mice, nude mice + T cells and nude mice + Tcells + anti-LtBR were quantified by ELISA in tissue homogenates (D). Gp38 expression in whole spleen of BALB/c, nude mice, nude mice + T cells and nude mice + Tcells + anti-LtBR detected by Western blotting (E). Data are representative of 5 mice per group.