Figure 4

Detection of anti- C-26 antibodies and anti-C-26 antibody cross-reactivity. (A) Anti- C-26 antibody levels in C-26 bearing-mice treated with RtH or HpH (pretreated before C-26 inoculation with RtH, or HpH, or not treated) were measured by ELISA. Mice inoculated with C-26 cells without Hcs or mice treated only with PBS were used as control groups. The results are presented as Relative Units (RU) calculated according to the titer of standard anti-C-26 antibodies used for ELISA. The data are presented as mean ± SD. p values are calculated using the Two-way ANOVA test (*p < 0.05; ***p < 0.001), in comparison to C-26 bearing mice. (B) ELISA assays for anti-C-26 antibody cross-reactivity. 96-well ELISA plates were coated with HpH or RtH (20 μg /ml) and after blocking the plates were incubated with diluted sera from mice inoculated with C-26 colon carcinoma cells only or PBS healthy controls. The results are presented as Relative Units (RU). Mean ± SD values were calculated for each group.