Figure 1
From: Vitamin D-binding protein controls T cell responses to vitamin D
Activated T cells express CYP27B1 and have the capacity to convert 25(OH)D 3 to 1,25(OH) 2 D 3 . (A) Relative CYP27B1 mRNA expression in T cells activated for 0-5 days normalized to CYP27B1 expression in naïve T cells. Values are given as mean + SEM from 3 independent experiments, *p < 0.05. (B) 1,25(OH)2D3 in the medium of T cells activated for 0-5 days in the presence of 100 nM 25(OH)D3. Data are given as mean ± SEM from 3 independent experiments, *p < 0.05. (C) Representative Western blot of VDR and CD3ζ (loading control) expression in T cells activated for 0-5 days.