Figure 2
From: Vitamin D-binding protein controls T cell responses to vitamin D
In the absence of serum activated T cells can produce 1,25(OH) 2 D 3 in concentrations that affect CD38 expression. Relative CD38 cell surface expression on T cells activated for 3 days in X-VIVO 15 (A) or X-VIVO 15 supplemented with 5% FBS (B) in the presence of the indicated concentrations of 25(OH)D3 and 1,25(OH)2D3 normalized to CD38 cell surface expression on T cells activated in the absence of 25(OH)D3 and 1,25(OH)2D3. Relative CD38 cell surface expression on T cells activated for 3 days in X-VIVO 15 medium supplemented with increasing concentrations of DBP (C) or albumin (D) in the presence of 100 nM 25(OH)D3 normalized to CD38 cell surface expression on T cells activated in the absence of 25(OH)D3. Relative CD38 cell surface expression on T cells activated for 3 days in the absence or presence of ketoconazole and the indicated concentrations of 25(OH)D3(E) or 1,25(OH)2D3(F) normalized to CD38 cell surface expression on T cells activated in the absence of 25(OH)D3 or 1,25(OH)2D3. (A-F) Mean values ± SEM from 3 independent experiments are plotted, *p < 0.05.