Figure 1
Comparison of intracellular and extracellular GrzB protein levels by memory CD4 and memory CD8 T cells. (A) Representative flow cytometry dotplots and mean ± sem intracellular GrzB protein expression by 1 × 105 untreated (UT) or activated (CD3/CD28 costimulation) memory T cells after 24 hrs culture (n = 10-13). (B) Representative ELISpot wells and mean ± sem GrzB spots (per 1 × 105 cells) of untreated or activated (CD3/CD28 costimulation) memory T cells after 24 hrs culture (n = 10-13). (C) Extracellular GrzB production (ELISA) by 1 × 105 untreated or activated (CD3/CD28 costimulation) memory T cells after 24 hrs culture (mean ± sem, n = 10-13).