Figure 2
Inter-assay correlations between intracellular and extracellular GrzB assays of memory T cells, and intra-donor correlations of GrzB production between memory T cells. (A-F) Spearman correlations between intracellular (flow cytometry) and extracellular (ELISA and ELISpot) GrzB measurements of 1 × 105 untreated (UT) or activated (CD3/CD28 costimulation) memory CD4 (A-C) or memory CD8 (D-F) T cells (n = 20-26). (G-I) Spearman correlations comparing GrzB production (costimulation values) of memory CD4 vs. memory CD8 T cells within single donors as measured by flow cytometry, ELISpot, or ELISA (n = 9-10).