Figure 4

Comparison of GrzB and perforin expression between memory CD4, memory CD8, and natural killer cells. (A) Representative GrzB/perforin dotplots of memory CD4, memory CD8, and NK cells. Cells were purified from peripheral blood, and 5 × 10⁵ cells (in 1 ml medium) were untreated (UT) or stimulated with IL2 (100 ng/ml) or anti-CD3 only mabs (1 μg/ml) for 24 hrs +/- brefeldin. (B) Mean (n = 4) distribution of perforin/GrzB subsets of memory CD4, memory CD8, and NK cells. (C-D) Extracellular GrzB and perforin production by memory CD4, memory CD8, and NK cells after 24 hrs culture +/- brefeldin (mean ± sem, n = 3-4, ^ap < 0.05 comparing UT NK cells to UT memory CD4 and memory CD8 T cells, ^bp < 0.05 comparing IL2-treated NK cells to IL2-treated memory CD4 and memory CD8 T cells, ^cp < 0.05 comparing UT or IL2-treated NK cells to UT or IL2-treated memory CD4 T cells).