The effect of Formoterol on the DC adoptive transfer model. BMDCs were stimulated with 10 μg/ml HDM in the presence or absence of 100 pM FORM for 24 hours. BMDCs were collected and washed twice with PBS, then 1.0 ± 106 BMDCs in 50 μl PBS were administered to naïve BALC/c mice intranasally. Control mice were administrated 1.0 ± 106 BMDCs without HDM and FORM stimulation (n = 6 in each group). On day 10, mice were challenged with 25 μg HDM. Twenty four hours later, mice were euthanized and BAL fluid and bronchial lymph nodes (LNs) were collected. (a) Total and differential cell counts in BAL fluids. After harvesting, bronchial lymph node cells were cultured in the presence of PMA and ionomycin. Concentrations of (b) IL-4, (c) IL-13, (d) IL-5 and (e) IL-17 in the supernatant were measured by ELISA. *p < 0.01, **p < 0.05.