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Figure 2 | BMC Immunology

Figure 2

From: Dichotomy in FcγRIIB deficiency and autoimmune-prone SLAM haplotype clarifies the roles of the Fc receptor in development of autoantibodies and glomerulonephritis

Figure 2

Female-biased production of anti-nuclear antibodies in RIIB−/−mice. (A) The total IgM and IgG levels in sera from different lines, including B6, RIIB−/−, SLAM129, and RIIB−/−SLAM129, at 24-28 weeks of age of both genders were measured by ELISA. For IgM and IgG determinations in male B6 mice, n =3 and 5, respectively; for other determinations, n ≥ 8. The data in each panel are from two separate measurements and are presented as means±S.D. *P <0.05; **P <0.01; ***P <0.001; ****P <0.0001; N.S., not significant. Student’s t-test. (B) Spontaneous production of ANAs by SLAM129 (n =16), RIIB−/− (n =12), RIIB−/−SLAM129 (n =12), and B6 (n =6) mice at 36 weeks (Upper) or 24 weeks (Lower) of age of both genders was measured in serum samples by HEp-2 staining combined with ELISA (Upper) and HEp-2-HEPANA Test (Lower), and expressed as the levels relative to that in sera collected from a 12-month-old female NZB/W F1 mouse, whose anti-dsDNA and anti-ssDNA levels were 6.64 g/ml and 4.69 g/ml, respectively. Horizontal lines represent the mean values. **P <0.01; ****P <0.0001; N.S., not significant. Student’s t-test. (C) IgG isotypes of ANAs in sera from RIIB−/− and SLAM129 mice of both genders. ANA IgG isotypes were determined by HEp-2 staining combined with ELISA, and presented as values relative to that in a 12-month-old NZB/W F1 control female mouse, as described in B.

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