CH-HG/OVA + GM-CSF immunization generated enhanced OVA-specific CD4+and CD8+T cell immune responses compared to control or CH-HG/OVA. C57BL/6 mice (5 per group) were immunized with CH-OVA/GM-CSF. (A) OVA-specific CD4+ T cell immune responses were assessed in the immunized mice. (B) The bar graph depicts the number of OVA-specific IL4-secreting CD4+ T cells per 3×105 splenocytes. (C) The bar graph depicts the number of IFN-γ producing OVA-specific CD8+ T cell precursors per 3×105 splenocytes from mice after immunization with OVA, CH-HG/OVA or CH-HG/OVA + GM-CSF. The data are presented as means±S.D. (n = 5), (*p <0.001).