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Figure 2 | BMC Immunology

Figure 2

From: Multiple factors influence the contribution of individual immunoglobulin light chain genes to the naïve antibody repertoire

Figure 2

Vκ10 promoter efficiency in pre-B cells. (A) Nucleic acid sequence comparison of the Vκ10 promoters. S1, S2 and S3 indicate the positions in the Vκ10C promoter that were mutated to the Vκ10A sequence. Mutated Vκ10 promoters were cloned into the PGL3 luciferase reporter vector containing the κ intronic enhancer (κen). Known regulatory sequences are in bold. PGL3κenAS, PGL3κenCS, PGL3κenS1, PGL3κenS2, PGL3κenS3 and PGL3κen were co-transfected with the control vector pCMV-β into (B) NFS-5 pre-B cells in 3 separate experiments (n = 15 for AS, CS, S1, S2, S3, n = 9 for κen) and (C) 18-81 pre-B cells in 7 separate experiments (n = 33 for AS, CS, S1, S2, S3, n = 17 for κen). Luciferase expression was assayed after 24 hours and differences in Vκ10A and Vκ10C promoter-driven expression of luciferase were compared by T-test. Error bars represent standard error of the mean. AS and CS are abbreviations for the Vκ10A and Vκ10C short/minimal promoter sequence.

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