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Figure 4 | BMC Immunology

Figure 4

From: Multiple factors influence the contribution of individual immunoglobulin light chain genes to the naïve antibody repertoire

Figure 4

Editing of Vκ10Jκ1 rearrangements by secondary Vκ inversion type rearrangements to Jκ2. (A) Schematic of Vκ10Jκ1 rearrangement displaced by subsequent inversional secondary Vκ rearrangement to Jκ2. Open boxes represent Vκ genes, black triangles next to open boxes represent Vκ RSS sequences, grey boxes represent Jκ genes and the back-to-back open and black triangle represent the back-to-back Jκ2 and Vκx RSS, retained on the genomic DNA. Arrows represent the PCR primers used to amplify the displaced Vκ10Jκ1 rearrangement. The upstream primer anneals in Vκ10 framework 1 to a sequence that is identical for all Vκ10 genes. The downstream primer was designed to include a portion of the Jκ2 23 bp spacer, the back-to-back Vκx/Jκ2 heptamers and part of the 12 bp space consensus sequence of other Vκ genes. (B) Percent of productive and nonproductive recombination products of displaced Vκ10A, Vκ10B and Vκ10C in BALB/c pre-B cells and B cells. The NP/P ratio is listed above each set of bars.

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