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Figure 3 | BMC Immunology

Figure 3

From: Carbon monoxide down-regulates α4β1 integrin-specific ligand binding and cell adhesion: a possible mechanism for cell mobilization

Figure 3

Effect of CO donor pretreatment on binding and dissociation of the LDV-FITC probe in cells stably transfected with CXCR4 and CXCR2, then treated with different Gαi-coupled receptor ligands. A. The experiment involved sequential addition of CORM-2 (75-150 μM, CO donor) or DMSO (control), the fluorescent LDV-FITC probe (4 nM), CXCL12/SDF-1 (12 nM), and excess unlabeled LDV competitor (1 μM). Rapid and reversible binding of the probe reflects the VLA-4 affinity change. B. The experiment involved sequential addition of CORM-2 (75μM, CO donor) or DMSO (control), the fluorescent LDV-FITC probe (4 nM), CXCL8/IL-8 (20 nM), and excess unlabeled LDV competitor (1 μM). A representative experiment of two independent experiments is shown.

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