Figure 2

Effect of VCGs on dendritic cell viability. The viability of BMDCs after incubation with VCGs was assessed using the XTT cell viability/proliferation assay. BMDCs cultured in 96-well flat-bottom plates (2 × 10⁴/well, BD Biosciences) were incubated with 50 μg/ml or 100 μg/ml of VCGs for 2 h. The XTT cell viability assay was performed 24 h later to investigate the toxicity of VCGs on BMDCs. Results are expressed as absorbance values of VCG-pulsed cells relative to media alone and the bars represent the mean and S.D. of three independent experiments. Significant difference between cultures incubated with media alone or cultures incubated with VCGs and Triton X-100 is indicated by asterisk (P** <0.001).