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Figure 3 | BMC Immunology

Figure 3

From: Vibrio cholerae ghosts (VCG) exert immunomodulatory effect on dendritic cells for enhanced antigen presentation and induction of protective immunity

Figure 3

Effect of VCG on activation marker expression by BMDCs using FACS analysis. Bone marrow-derived DCs (BMDCs) were isolated from mice by established procedures using IL-4 and GM-CSF and characterized as loosely adherent mononuclear cells expressing high levels of CD11c but lacking B220 surface antigens. Harvested cells were pulsed with VCGs or UV-EBs or UV-EBs + VCGs for 24 h, stained with conjugated monoclonal antibodies against CD11C, CD40, CD80, CD83, CD86, 1Ab, CD14 and CD71 or isotype-matched controls, and quantified in triplicate by flow cytometry. The data shows the mean fluorescence intensity of staining (A) and percentage of cells (B) expressing the indicated marker after treatment with antigens (unshaded area) or culture media (Blue shaded area). Data is from one of two experiments with similar results.

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