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Figure 4 | BMC Immunology

Figure 4

From: Vibrio cholerae ghosts (VCG) exert immunomodulatory effect on dendritic cells for enhanced antigen presentation and induction of protective immunity

Figure 4

Effect of VCG on the production of Th1/Th2 promoting cytokines by BMDCs. BMDCs were pulsed ex vivo with UV-EBs, UV-EB + VCG, or medium for 24 h. Culture supernatants were collected and assayed for IL-12, TNF-α, IL-10 and IL-4 cytokines by cytokine ELISA using the Bio-Plex cytokine assay kit in combination with the Bio-Plex Manager software. The concentration of the cytokines in each sample was obtained by extrapolation from a standard calibration curve generated simultaneously. Data are shown as the mean values (± S.D.) for triplicate cultures for each experiment. The results are from two independent experiments. Statistically significant differences between DCs alone and treatment groups was evaluated at (p* < 0.05) or (p** < 0.01).

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BMC Immunology

ISSN: 1471-2172

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