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Figure 2 | BMC Immunology

Figure 2

From: Lipid raft-based membrane order is important for antigen-specific clonal expansion of CD4+ T lymphocytes

Figure 2

Reversibility of effects of 7-KC on abolishing FRET between Thy-1 and CD3ε present on membrane rafts on CD4+T cells from the lymph node. FRET between Thy-1 and CD3ε molecules was established using a donor and acceptor pair of anti-CD3ε PE and anti-Thy1 AlexaFluor 647 respectively after CD4+ cells were treated with a range of 7-KC concentrations for 4 hrs or left untreated. Reversibility of FRET was assessed after washing excess 7-KC and re-culturing cells for an additional 20 hrs. A representative experiment of three independent replicates is shown.

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