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Figure 2 | BMC Immunology

Figure 2

From: ImmunoGlobulin galaxy (IGGalaxy) for simple determination and quantitation of immunoglobulin heavy chain rearrangements from NGS

Figure 2

Outline of IGGalaxy repertoire analysis and reporting. Starting at the “Trimmed FASTA”, the user can use IMGT/HIGH-QUEST (Down) or igBLAST (Up) to perform the alignment of the sequences for further analysis in IGGalaxy. When alignment is done, the user uploads the IMGT/HIGH-QUEST result and converts it into the proprietary format or uses the igBLASTparser IGGalaxy tool to do the same for the igBLAST output. After that, the remaining steps are the same for both (or any other alignment tool that is implemented). The Experimental Design step combines several samples together for easy analysis. Finally the Reporting tool generates a report on the experiment.

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BMC Immunology

ISSN: 1471-2172

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