Figure 2
From: Adoptive T-cell therapy of prostate cancer targeting the cancer stem cell antigen EpCAM
Human PBLs transduced with retroviruses encoding the EpCAM-specific CAR. A) A diagram of the EpCAM-specific CAR, including, VH, anti-human EpCAM immunoglobulin heavy chain variable region; VL, anti-human EpCAM immunoglobulin light chain variable region; CD28, consisting of part of the extracellular region and the entire transmembrane and intracellular regions of CD28, and CD3ζ, the cytoplasmic region only. B) PBLs were stimulated with OKT3 and anti-human CD28 antibodies for 2 d before being transduced with retroviruses encoding the EpCAM-specific CAR. Five days later, the PBL phenotypes were examined by staining with anti-CD3, anti-CD8, and anti-CD4 antibodies. C) Transduction efficiency was determined by staining with biotinylated protein L and PE conjugated streptavidin. PBLs not transduced were used as a negative control.