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Table 2 T1/T2-stimulatory activity of IFN-α-DCs

From: Elevated levels of dehydroepiandrosterone as a potential mechanism of dendritic cell impairment during pregnancy

  Culture conditions Intracellular expression and production of the cytokines IFN-γ and IL-4
   Control group (n = 10) NP women (n = 8) HA women (n = 8)
   Value SI Value SI Value SI
CD3+ IFN-γ+ (%) 0 1.5 ± 0.2   1.8 ± 0.5   1.8 ± 0.5  
  + DCs 5.9 ± 0.6 5.2 ± 0.9 1.5 ± 0.27** 1.1 ± 0.2** 2.5 ± 0.3**# 2.0 ± 0.4**#
CD3+ IL-4+ (%) 0 1.7 ± 0.1   2.6 ± 0.4   3.3 ± 0.5  
  + DCs 2.1 ± 0.4 1.3 ± 0.2 8.6 ± 1.0** 3.5 ± 0.4** 7.4 ± 1.0** 2.4 ± 0.5*
IFN-γ (pg/ml) 0 18.2 ± 5.7   16.5 ± 2.8   16 ± 3.0  
  + DCs 353 ± 41 22.2 ± 3.1 63.4 ± 27.8** 3.4 ± 0.9** 218 ± 69* # 13.5 ± 3.1*#
IL-4 (pg/ml) 0 1.0 ± 0.08   1.0 ± 0.09   1.0 ± 0.12  
  + DCs 2.0 ± 1.0 2.0 ± 1.0 4.6 ± 2.2 4.6 ± 2.2 5.4 ± 2.0* 5.4 ± 2.0*
  1. Donor MNCs were co-cultured with allogenic IFN-α-DCs from non-pregnant women (control), normal pregnant women (NP), or hyperandrogenia pregnant women (HA) for 72 h at an MNC-to-DC ratio of 10:1. For the final 18 h of culture, 10 μg/ml Brefeldin A was added. Intracellular expression of IFN-γ and IL-4 was detected within the CD3+ gate by flow cytometry. To determine the levels of cytokine production by MNCs, cell-free supernatants were collected after 5 days of co-culturing donor MNCs and allogenic IFN-α-DCs from the control group, NP-women, or HA-women (at a MNC-to-DC ratio of 10:1) and were assessed for production of IL-4 and IFN-γ using specific ELISA kits. Data are presented as mean (± SE) cytokine concentrations (pg/ml) or the percentage of CD3+ cells from the indicated number of independent experiments. SI indicates the stimulation index, which was calculated as the ratio of cytokine concentrations (pg/ml) or intracellular cytokine expression (%) by MNCs in the presence or absence of DCs. *, pU < 0.05, **, pU < 0.01, compared to the control women group; #, pU < 0.05, compared to the NP-women group. Significant differences were detected using the Mann–Whitney U test.