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Figure 3 | BMC Immunology

Figure 3

From: LPS stimulation of purified human platelets is partly dependent on plasma soluble CD14 to secrete their main secreted product, soluble-CD40-Ligand

Figure 3

Platelet sCD40L production and secretion. Platelets were unstimulated or stimulated with TRAP (50 μg/ml, 30 min at RT). Platelets from platelet-rich plasma (PRP; black bars), or from platelet suspensions with added anti-human CD14 (clone 18D11 20 μg/ml; grey bars) or isotype control (clone 15H6; white bars) were tested. Platelets were unstimulated or stimulated with E. coli LPS (1–10 μg/ml; 30 min at RT). Mean sCD40L release was 1.2 ng/ml for unstimulated PRP and 1.5, 1.9, 2.2 and 3.1 ng/ml for LPS stimulation at concentrations of 1, 2, 3 and 10 μg/ml, respectively. Data are expressed as fold increase of controls (± SD; n = 10 independent experiments). *P < 0.05 (Wilcoxon paired test; stimulated vs. non-stimulated).

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