Figure 8

Effect of MAb 5261-muIg on adoptively transferred EAE in SJL/J mice. Mice were treated i.p. with 30 mg/kg of antibody starting on the day of the adoptive transfer and twice a week thereafter for the total of 4 weeks (n = 10 per group). (A) MAb 5261-muIg in Th17-SJL EAE. (B) MAb 5261-muIg in Th17 vs. Th1 SJL-EAE: Th17 EAE: GMS +/− SE (GMS calculated from three independent experiments); Th1 EAE: GMS +/− SD (Results of one experiment +/− SD). (C) MAb 5261-muIg effect on muCXCL13-induced migration of Th17 and Th1 cells from successful SJL/J passive EAE transfer experiments (concentrations: 5 μg/ml muCXCL13; 50 μg/ml of each antibody). Results are presented as average of triplicate measurements +/− SE (except for Th1 cell migration data which originated from a single measurement). Statistical significance was evaluated using unpaired Student’s t-test (A) and one-way ANOVA followed by Bonferroni’s multiple comparison post test (B and C). **** P < 0.0001; *P < 0.1.