Figure 1

The impact of Wnt/β-catenin signaling on necrosis of RAW264.7 cells in response to BCG infection. (A) Representatives of scatter dot plot of flow cytometric analysis for the necrosis cell fraction for RAW264.7 cells treated with different doses of BCG. (B) A dose-dependent cell necrotic death of RAW264.7 cells infected with indicated dose of BCG for 6 h. The cell necrosis fraction was ascertained by a flow cytometry assay. (C) A time-dependent cell necrotic death of RAW264.7 cells infected with BCG. RAW264.7 cells were infected with BCG at MOI of 10 for indicated time. The cell necrosis fraction was ascertained by a flow cytometry assay. (D) Representatives of scatter dot plot of flow cytometric analysis for the necrosis cell fraction for RAW264.7 cells treated with indicated conditions for 6 h. The cells distributed in upper two quadrants were PI positive cells and considered as necrotic cell fraction. (E) Wnt/β-catenin signaling inhibits BCG-induced cell necrosis. RAW264.7 cells were infected with BCG at MOI of 10 or indicated conditions for 6 h prior to be used for flow cytometric analysis. Quantitative results revealed that an activation of Wnt/β-catenin signaling could inhibit cell necrosis, regardless of BCG infection. Compared to a Control-CM treated control cells, *: p < 0.05; **: p < 0.01. A control-CM treated compared to its corresponding Wnt3a-CM treated cells, #: p < 0.05; ##: p < 0.01, Data represented the mean ± SD from three independent triplicated experiments (N = 9).