Morphological analysis of the impact of Wnt3a on BCG-infected RAW264.7 cells necrosis. RAW264.7 cells were exposed to Wnt3a-CM or control-CM, followed by infection of BCG at MOI of 10 for 24 h prior to be employed for EM analysis. (A) Representative images of TEM (top panel) and SEM (bottom panel) of healthy RAM264.7 cells (left panel) and necrotic cells (right panel). (B) Quantitative analysis of cells with a necrotic phenotype as determined by morphology using EM images. Control-CM treated cells compared to its corresponding Wnt3a-CM treated cells, #: p < 0.05; ##: p < 0.01. Data represented the mean ± SD from three independent triplicated experiments (N = 9). Bar in SEM images = 2 μm; Bar in TEM images = 10 μm.